rna transcriptase
英
美
RNA转录酶
双语例句
- Given the properties of reverse transcriptase and RNA replicase, can you suggest a reason for the small size of these viral genomes?
指出逆转录酶和RNA复制酶的特点，你能提出一个理由来说明这些病毒基因组为什么这么小吗？ - Hydroxyapatite Nanoparticles Mediated Human Telomerase Reverse Transcriptase RNA Interference of Lung Cancer in Vitro
纳米羟基磷灰石介导靶向端粒酶逆转录酶RNA干扰的肺癌基因治疗的实验研究 - The reverse transcriptase isolated from human leukaemic cells resembles that from RNA tumour viruses in several respects.
从人白血病细胞分离得到的递向转录酶在如下几方面类似于RNA肿瘤病毒逆向转录酶。 - The M genome RNA segment of epidemic hemorrhagic fever virus R22 strain was characterized by molecular cloning with reverse transcriptase and polymerase chain reaction ( RT-PCR) techniques, and the nucleotide sequences of the corresponding cDNA clones were determined.
用标准的cDNA合成与多聚酶链式反应（PCR）相结合的方法，克隆了我国家鼠型出血热病毒R22株M片段cDNA，完成了全部核苷酸序列分析。 - Telomerase is a special reverse transcriptase enzyme, which uses its own RNA component as a template to elongate the 3 '-single-strand DNA extension of the chromosome terminus by the sythesis of telomeric repeats.
端粒酶是一类特殊的核糖核蛋白酶，能够以自身的RNA为模板，通过自身的催化亚单位作用逆转录合成端粒，添加到染色体末端，维持染色体端粒末端结构的稳定性。 - Expression of Telomerase RNA and Telomerase Reverse Transcriptase mRNA in Lesions of Psoriasis Vulgaris
寻常性银屑病皮损端粒酶RNA、反转录酶mRNA表达 - Detection of Hantavirus by reverse transcriptase semi nested polymerase chain reaction and its preliminary application
逆转录-半套式聚合酶链反应检测汉坦病毒RNA及其临床初步应用 - Objective: To study the effect of short hairpin RNA ( shRNA) on the expression of human telomerase reverse transcriptase ( hTRT) and telomerase activity in PC-3M cells.
目的：研究短发夹状RNA（shRNA）对PC3M细胞中端粒酶逆转录酶（hTRT）基因表达及端粒酶活性的影响。 - Methods Total RNA in the metastatic lymph nodes from patients with colorectal cancer was extracted routinely, and reverse transcriptase PCR employed to amplify the heavy chain Fd and light chain κ cDNA.
方法取大肠癌病人转移淋巴结，提取淋巴结总RNA，逆转录PCR扩增重链Fd和K轻链cDNA。 - Telomerase is a specialized reverse transcriptase that elongates the single-stranded chromosome ends and oligonucleotides in vivo and in vitro.
端粒酶是一种特殊的逆转录酶，它能在体内延伸染色体单链末端，在体外延伸寡聚核苷酸。一般认为端粒酶的蛋白催化亚基和RNA亚基组成了核心酶，它们对体外活性是必需的。 - Methods Synovial and cartilage tissue samples obtained from knee joint during operation in patients with RA were examined for telomerase activity ( including human telomerase RNA, i. e., hTR and human telomerase reverse transcriptase, i. e., hTERT) using the in situ hybridization technique.
方法在类风湿性关节炎患者的膝关节手术中获得软骨及沿膜标本，通过原位杂交的方法，检测端粒酶（包括人端粒酶RNA和人端粒酶反转录酶）的活性。 - Last, the tiny amount RNA was extracted from atrial cells captured by LCM, and the genes of β-actin and GAPDH were amplified by reverse transcriptase polymerase chain reaction ( RT-PCR).
最后进行LCM分离纯化心房细胞，通过逆转录-聚合酶链反应（RT-PCR）扩增β-actin及GAPDH基因。 - An RNA transcriptase activity of grass carp hemorrhage virus cores was demonstrated by the study on the in vitro transcription with α-32P ATP as the label.
以α-~3~2P-ATP为标记物研究了草鱼出血病病毒的体外转录，证实该病毒的核心具有RNA转录酶。 - Forth, make inverse transcription parallel experiment with difference inverse transcriptase, it is easy to distinguish from false-positive process in RNA process in experiment, today, Northern blot is better method on false-positive of identification, some peoples who have result well by improved correctly.
4用两种不同的反转录酶进行反转录平行实验，很容易区分出RNA制备过程中造成的假阳性，目前假阳性鉴定大多还是以Northern印迹为基础，再作些适当的改进。 - The RNA was isolated from the virions of tobacco mosaic virus ( Guangdong tobacco strain) and was used to convert to synthesize first-strand cDNA with AMV reverse transcriptase and the synthetic primer.
根据已报道的烟草花叶病毒移动蛋白基因序列，设计合成一对特异性引物。以广东TMV株系RNA为模板，用AMV反转录酶反转录合成cDNA第一链； - The core enzyme actually consists of two components: an RNA, which serves as template for the simple sequence tracts, and a catalytic subunit of telomerase that acts as a reverse transcriptase on the RNA template.
多数真核细胞的端粒酶能将单一重复序列加到端粒DNA的3′末端.端粒酶主要由模板RNA和端粒酶蛋白催化亚基组成，后者以前者为模板起逆转录酶的作用。 - Methods Virion RNA was transcribed into cDNA by reverse transcriptase, cDNA amplified by PCR, the productions of PCR were purified.
方法病毒粒RNA经逆转录合成cDNA，经聚合酶链反应（PCR）扩增，产物纯化，采用双脱氧链末端终止法进行核苷酸序列测定。 - Objective To investigate whether telomerase activity, human telomerase RNA ( HTR) and human telomerase reverse transcriptase ( HTERT) expression were associated with tumor development in lung cancer and whether telomerase is regulated at gene level or transcriptional level.
目的探讨hTR、hTERT与端粒酶活性的表达是否与肺癌的发生发展有关，深入了解端粒酶基因对端粒酶活性的调控是在基因水平还是在转录水平。 - Methods RNA was extracted from rat model of the focal cerebral ischemia reperfusion and mRNA expression levels of TNF α, IL β and ICAM 1 at various time points after reperfusion were measured by semiquantitative reverse transcriptase polymerase chain reaction ( RT PCR).
方法提取局灶性脑缺血再灌注大鼠的RNA，用半定量逆转录多聚酶链反应（RTPCR）测定再灌注不同时间点TNFα，ILβ及ICAM1在mRNA的表达水平。 - Reverse transcriptase Extra-cellular domain gene was amplified by PCR, and cloned to the pGEM~ T easy plasmids vector.
以胚肝RNA逆转录产物为模板，PCR扩增TβRⅡ胞外区基因，克隆到pGEM-Teasy质粒载体上。 - Objection: Telomerase, a unique ribonucleoprotein with reverse transcriptase activity, and its RNA component acts as the template and the telomeric DNA sequences synthesized by telomerase.
目的：端粒酶是一种具有逆转录酶活性的核糖核蛋白酶，它能以自身RNA为模板，合成端粒DNA序列。 - Influence of RNA interference targeting against human telomerase reverse transcriptase on expression of C-myc protein
抑制端粒酶逆转录酶基因表达对C-myc蛋白表达的影响 - The reverse transcriptase ( RT) plays a crucial role for the replication of HIV type-1 ( HIV-1) in the virus life cycle, and is responsible for the convertion of the single-stranded RNA viral genome into double-stranded DNA that subsequently integrates into the host DNA.
HIV-1逆转录酶（reversetranscriptaseRT）在病毒生命周期中起重要作用。病毒通过逆转录酶将单链RNA逆转录为双链DNA，随后病毒DNA迅速整合到宿主DNA中，从而完成转录、翻译等整个生命周期。 - Direct reverse transcriptase in situ polymerase chain reaction for detection of viral RNA in the brain tissues from suckling mice experimentally infected with hantavirus: a comparison with in situ hybridization
直接逆转录原位PCR检测实验性汉坦病毒感染乳鼠脑组织中病毒RNA及其与原位分子杂交的比较 - Extraction of total RNA from culture clones which were expanded, and then detecting the target gene was integrated into the genome of HeLa cells through reverse transcriptase PCR method ( RT-PCR).
对单克隆进行扩大培养，提取其总RNA，逆转录酶PCR法（Reversetranscriptase-PCR，RT-PCR）检测到目的基因已整合到HeLa细胞基因组中，说明重组质粒在细胞中稳定表达。 - Background: Telomerase is a specialized reverse transcriptase responsible for synthesizing telomeric DNA at the ends of chromosomes.
研究背景：端粒酶是一种特殊的反转录酶，能以自身的RNA为模板反转录合成端粒DNA从而维持染色体末端的稳定。 - Telomerase is a ribonucleic acid ( RNA)-protein complex and a specialized type of reverse transcriptase, which synthesizes telomeric DNA onto chromosomal ends by using its RNA component as a template and contributes to chromosomal stability.
端粒酶（telomerase）是核糖核酸和蛋白质的复合体，是一种专一的逆转录酶，主要功能是以自身RNA为模板，从头合成端粒，维持染色体末端稳定。 - Reverse transcription was performed with hot-resistance reverse transcriptase and random primers.
逆转录：利用耐热性逆转录酶和随机引物，逆转录各成员的RNA。 - Given no proofreading function of RNA polymerase and reverse transcriptase, the retroelements are of high variability.
由于RNA聚合酶和逆转录酶均无校对功能，就使得逆转座子具有高度变异性。